Androgen Receptor 抗体 (N-Term)
Quick Overview for Androgen Receptor 抗体 (N-Term) (ABIN185266)
抗原
See all Androgen Receptor (AR) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- Androgen Receptor
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序列
- EVQLGLGRVY PRPPSC
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特异性
- This antibody is expected to recognize isoform 1 (NP_000035.2) only.
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预测反应
- Human, Mouse, Rat, Dog, Pig, Cow
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2.
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亚型
- IgG
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anti-Androgen Receptor (AR) antibody
AR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Androgen Receptor (AR) (N-Term) antibodyAR 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Androgen Receptor (AR) antibodyAR 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated
anti-Androgen Receptor (AR) antibodyAR 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Androgen Receptor (AR) (AA 491-679) antibodyAR 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Androgen Receptor (AR) (AA 221-320) antibodyAR 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-Androgen Receptor (AR) (AA 15-246) antibodyAR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Androgen Receptor (AR) (AA 365-392) antibodyAR 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Androgen Receptor (AR) (AA 825-919) antibodyAR 适用: 人, 小鼠, 大鼠, Pig, 山羊 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Androgen Receptor (AR) (pSer578) antibodyAR 适用: 人, 小鼠, 大鼠 IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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DS WB Results: Approx 100 kDa band observed in Human Cerebellum lysates (calculated MW of 99.2 kDa according to NP_000035.2). Recommended concentration:0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
DS IHC Results: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.
Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Androgen Receptor (AR)
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别名
- Androgen Receptor
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背景
- AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1
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基因ID
- 367
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NCBI登录号
- NP_000035
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途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding
抗原
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