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IFT74 抗体 (C-Term)

There are 2+ publications for this product available. The 山羊 多克隆 anti-IFT74 antibody is suitable to detect IFT74 in samples from 人. It has been validated for ELISA 和 WB.
产品编号 ABIN185213
发货至: 中国
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Quick Overview for IFT74 抗体 (C-Term) (ABIN185213)

抗原

See all IFT74 抗体
IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This IFT74 antibody is un-conjugated

应用范围

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ELISA, Western Blotting (WB)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    CMG1 / CCDC2 / IFT74

    序列

    KTIVDALHST SGN

    交叉反应

    人, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2 kDa according to AAK77221.1). The observed molecular weight corresponds to earlier findings in literature (Momeni et al, BMC Neurol. 2006 Dec 13,6:44., PMID: 17

    Peptide ELISA: antibody detection limit dilution 1:32000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Boldt, Mans, Won, van Reeuwijk, Vogt, Kinkl, Letteboer, Hicks, Hurd, Naggert, Texier, den Hollander, Koenekoop, Bennett, Cremers, Gloeckner, Nishina, Roepman, Ueffing: "Disruption of intraflagellar protein transport in photoreceptor cilia causes Leber congenital amaurosis in humans and mice." in: The Journal of clinical investigation, Vol. 121, Issue 6, pp. 2169-80, (2011) (PubMed).

    Momeni, Schymick, Jain, Cookson, Cairns, Greggio, Greenway, Berger, Pickering-Brown, Chiò, Fung, Holtzman, Huey, Wassermann, Adamson, Hutton, Rogaeva, St George-Hyslop, Rothstein, Hardiman, Grafman et al.: "Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. ..." in: BMC neurology, Vol. 6, pp. 44, (2007) (PubMed).

  • 抗原

    IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))

    别名

    IFT74

    背景

    IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2, CMG1

    基因ID

    80173, 313365

    NCBI登录号

    NP_001092692, NP_001092693, NP_079379

    途径

    Hedgehog Signaling, Chromatin Binding
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