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POLL 抗体 (C-Term)

This 山羊 多克隆 antibody specifically detects POLL in WB 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN185143
发货至: 中国
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Quick Overview for POLL 抗体 (C-Term) (ABIN185143)

抗原

See all POLL 抗体
POLL (Polymerase (DNA Directed), lambda (POLL))

适用

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宿主

  • 42
山羊

克隆类型

  • 42
多克隆

标记

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This POLL antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    POLL / POLkappa

    序列

    LGLPYREPAE RDW

    特异性

    It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-LGLPYREPAERDW, from the C Terminus of the protein sequence according to NP_037406.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5 kDa according to Human NP_037406.1). Recommended concentration: 0.5-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    POLL (Polymerase (DNA Directed), lambda (POLL))

    别名

    POLL

    背景

    POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA

    基因ID

    27343, 56626

    NCBI登录号

    NP_037406

    途径

    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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