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ARHGEF18 抗体 (C-Term)

Verified ARHGEF18 适用: 人 ELISA, WB, IF 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN185123
发货至: 中国
  • 抗原 See all ARHGEF18 抗体
    ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
    抗原表位
    • 15
    • 8
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    C-Term
    适用
    • 46
    • 4
    • 1
    宿主
    • 44
    • 2
    山羊
    克隆类型
    • 44
    • 2
    多克隆
    标记
    • 15
    • 6
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARHGEF18 antibody is un-conjugated
    应用范围
    • 27
    • 26
    • 13
    • 13
    • 8
    • 6
    • 3
    • 3
    • 3
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF)
    原理
    P114-RHO-GEF / ARHGEF18
    序列
    SAKEDASKED VIFF
    特异性
    This antibody is expected to recognize both reported isoforms (NP_056133.2, NP_001124427.1).
    交叉反应
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2, NP_001124427.1.
    亚型
    IgG
    Top Product
    Discover our top product ARHGEF18 Primary Antibody
  • 应用备注

    Western Blot: Approx 140+90 kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114 kDa according to NP_056133.2 and 131 kDa accoeding to NP_001124427.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This anti

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Zaritsky, Tseng, Rabadán, Krishna, Overholtzer, Danuser, Hall: "Diverse roles of guanine nucleotide exchange factors in regulating collective cell migration." in: The Journal of cell biology, Vol. 216, Issue 6, pp. 1543-1556, (2017) (PubMed).

    Herder, Swiercz, Müller, Peravali, Quiring, Offermanns, Wittbrodt, Loosli: "ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation." in: Development (Cambridge, England), Vol. 140, Issue 13, pp. 2787-97, (2013) (PubMed).

    Terry, Elbediwy, Zihni, Harris, Bailly, Charras, Balda, Matter: "Stimulation of cortical myosin phosphorylation by p114RhoGEF drives cell migration and tumor cell invasion." in: PLoS ONE, Vol. 7, Issue 11, pp. e50188, (2012) (PubMed).

    Terry, Zihni, Elbediwy, Vitiello, Leefa Chong San, Balda, Matter: "Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis." in: Nature cell biology, Vol. 13, Issue 2, pp. 159-66, (2011) (PubMed).

  • 抗原
    ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
    别名
    ARHGEF18 (ARHGEF18 产品)
    别名
    arhgef18 antibody, wu:fc21e04 antibody, wu:fj05f11 antibody, zgc:85686 antibody, P114-RhoGEF antibody, AI467246 antibody, D030053O22Rik antibody, rho/rac guanine nucleotide exchange factor (GEF) 18a antibody, Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody, Rho/Rac guanine nucleotide exchange factor 18 antibody, rho guanine nucleotide exchange factor 18 antibody, rho/rac guanine nucleotide exchange factor (GEF) 18 antibody, arhgef18a antibody, ARHGEF18 antibody, LOC100027283 antibody, Arhgef18 antibody
    背景
    ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18
    基因ID
    23370
    NCBI登录号
    NP_056133, NP_001124427
    途径
    Neurotrophin Signaling Pathway
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