ARHGEF18 抗体 (C-Term)
(4 references)Quick Overview for ARHGEF18 抗体 (C-Term) (ABIN185123)
抗原
See all ARHGEF18 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- P114-RHO-GEF / ARHGEF18
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序列
- SAKEDASKED VIFF
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特异性
- This antibody is expected to recognize both reported isoforms (NP_056133.2, NP_001124427.1).
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交叉反应
- 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2, NP_001124427.1.
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亚型
- IgG
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anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) (Center) antibody
ARHGEF18 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) (AA 159-551) antibodyARHGEF18 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) (AA 1-89) antibodyARHGEF18 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 8H6 unconjugated
anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) (AA 159-551) antibodyARHGEF18 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) antibody (HRP)ARHGEF18 适用: 人, 犬 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) antibody (Biotin)ARHGEF18 适用: 人, 犬 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18) antibody (FITC)ARHGEF18 适用: 人, 犬 ELISA, WB, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
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应用备注
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Western Blot: Approx 140+90 kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114 kDa according to NP_056133.2 and 131 kDa accoeding to NP_001124427.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This anti
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "Diverse roles of guanine nucleotide exchange factors in regulating collective cell migration." in: The Journal of cell biology, Vol. 216, Issue 6, pp. 1543-1556, (2017) (PubMed).
: "ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation." in: Development (Cambridge, England), Vol. 140, Issue 13, pp. 2787-97, (2013) (PubMed).
: "Stimulation of cortical myosin phosphorylation by p114RhoGEF drives cell migration and tumor cell invasion." in: PLoS ONE, Vol. 7, Issue 11, pp. e50188, (2012) (PubMed).
: "Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis." in: Nature cell biology, Vol. 13, Issue 2, pp. 159-66, (2011) (PubMed).
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: "Diverse roles of guanine nucleotide exchange factors in regulating collective cell migration." in: The Journal of cell biology, Vol. 216, Issue 6, pp. 1543-1556, (2017) (PubMed).
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- ARHGEF18 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 18 (ARHGEF18))
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别名
- ARHGEF18
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背景
- ARHGEF18, Rho/Rac guanine nucleotide exchange factor (GEF) 18, P114-RHO-GEF, KIAA0521, MGC15913, Rho-specific guanine nucleotide exchange factor p114, P114-RhoGEF, Rho/Rac guanine nucleotide exchange factor 18
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基因ID
- 23370
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NCBI登录号
- NP_056133, NP_001124427
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途径
- Neurotrophin Signaling Pathway
抗原
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