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UBA2 抗体 (N-Term)

This 山羊 多克隆 antibody specifically detects UBA2 in WB, ELISA 和 IF. It exhibits reactivity toward 人 和 小鼠.It has been mentioned in 1 publication
产品编号 ABIN185083
发货至: 中国
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Quick Overview for UBA2 抗体 (N-Term) (ABIN185083)

抗原

See all UBA2 抗体
UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

适用

  • 61
  • 26
  • 23
  • 3
  • 2
  • 1
  • 1
  • 1
人, 小鼠

宿主

  • 55
  • 7
山羊

克隆类型

  • 37
  • 25
多克隆

标记

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  • 4
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  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This UBA2 antibody is un-conjugated

应用范围

  • 48
  • 31
  • 24
  • 17
  • 16
  • 14
  • 7
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 8
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term

    原理

    SAE2 / UBA2

    序列

    ALSRGLPREL AEA

    交叉反应

    Cow, 人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    ALSRGLPRELAEA-C

    亚型

    IgG
  • 应用备注

    Western Blot: This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013,77(7):1575-8. PMID: 23832333.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Kanemaru, Saitoh: "High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system." in: Bioscience, biotechnology, and biochemistry, Vol. 77, Issue 7, pp. 1575-8, (2013) (PubMed).

  • 抗原

    UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

    别名

    SAE2

    背景

    UBA2, SAE2, SUMO-1 activating enzyme subunit 2

    基因ID

    10054, 50995

    NCBI登录号

    NP_005490
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