UBA2 抗体 (N-Term)
Quick Overview for UBA2 抗体 (N-Term) (ABIN185083)
抗原
See all UBA2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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原理
- SAE2 / UBA2
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序列
- ALSRGLPREL AEA
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交叉反应
- Cow, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- ALSRGLPRELAEA-C
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亚型
- IgG
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应用备注
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Western Blot: This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013,77(7):1575-8. PMID: 23832333.
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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: "High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system." in: Bioscience, biotechnology, and biochemistry, Vol. 77, Issue 7, pp. 1575-8, (2013) (PubMed).
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: "High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system." in: Bioscience, biotechnology, and biochemistry, Vol. 77, Issue 7, pp. 1575-8, (2013) (PubMed).
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- UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))
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别名
- SAE2
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背景
- UBA2, SAE2, SUMO-1 activating enzyme subunit 2
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基因ID
- 10054, 50995
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NCBI登录号
- NP_005490
抗原
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