BOLL 抗体 (C-Term)
Quick Overview for BOLL 抗体 (C-Term) (ABIN185072)
抗原
See all BOLL 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- BOULE / BOLL
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序列
- QPEPIKTVWS IHY
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特异性
- This antibody is expected to recognize both reported isoforms (NP_932074.1, NP_149019.1).
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交叉反应
- Cow, 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-QPEPIKTVWSIHY, from the C Terminus of the protein sequence according to NP_932074.1, NP_149019.1.
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亚型
- IgG
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anti-Bol, Boule-Like (BOLL) (AA 1-295) antibody
BOLL 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Bol, Boule-Like (BOLL) (AA 185-283) antibodyBOLL 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Bol, Boule-Like (BOLL) (AA 185-283) antibodyBOLL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5B8 unconjugated
anti-Bol, Boule-Like (BOLL) (AA 80-176) antibodyBOLL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-Bol, Boule-Like (BOLL) (AA 185-283) antibodyBOLL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5H8 unconjugated
anti-Bol, Boule-Like (BOLL) (AA 80-176) antibodyBOLL 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 2G4 unconjugated
anti-Bol, Boule-Like (BOLL) (AA 80-176) antibodyBOLL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C3 unconjugated
anti-Bol, Boule-Like (BOLL) (AA 1-283) antibodyBOLL 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bol, Boule-Like (BOLL) (AA 38-87) antibodyBOLL 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining in spermatocytes, but not in spermatogonia. Recommended concentration, 4-6 μg/mL.
Western Blot: Preliminary experiments gave an approx 22 kDa band in Human Testis lysates after 1.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.
Peptide ELISA: antibody detection limit dilution 1:32000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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