STRADB 抗体 (C-Term)
Quick Overview for STRADB 抗体 (C-Term) (ABIN184938)
抗原
See all STRADB 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- ALS2CR2 / ILPIP
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序列
- CDFPDEKDSY WEF
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特异性
- This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).
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交叉反应
- Cow, 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence according to NP_061041.2, AAF71042.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration: 3-5 μg/mL.
Western Blot: Approx 35 kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0 kDa according to NP_061041.2 (alpha isoform) and 31.2 kDa according to AAF71042.1 (beta isoform). In transfected HEK293 transiently expressing ILPIP a
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U251 cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- STRADB (STE20-Related Kinase Adaptor beta (STRADB))
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别名
- STRADB
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背景
- STRADB, ALS2CR2, ILPIP, PAPK, ILPIPA, CALS-21, PRO1038, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, pseudokinase ALS2CR2
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基因ID
- 55437
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NCBI登录号
- NP_061041
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途径
- AMPK Signaling
抗原
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