ACSL4 抗体 (C-Term)
Quick Overview for ACSL4 抗体 (C-Term) (ABIN184846)
抗原
See all ACSL4 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- FACL4 / ACSL4
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序列
- HYLKDIERMY GGK
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特异性
- This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-HYLKDIERMYGGK, from the C Terminus of the protein sequence according to NP_004449.1, NP_075266.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Small Intestine and Brain (Cortex). Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 80-85 kDa band observed in lysates of cell line HepG2 and approx 75 kDa in preliminary testing of Human Adipose lysate (calculated MW of 79.2 kDa according to NP_075266.1 and 74.4 kDa according to NP_004449.1). Recommended concentration:
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Golgi and Endoplasmic reticulum of HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ACSL4 (Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4))
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别名
- ACSL4
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背景
- ACSL4, acyl-CoA synthetase long-chain family member 4, FACL4, fatty-acid-Coenzyme A ligase, long-chain 4, ACS4, LACS 4, lignoceroyl-CoA synthase, Fatty acid coenzyme A ligase, long-chain 4, MRX63, LACS4, MRX68, acyl-CoA synthetase 4, long-chain fatty-acid
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基因ID
- 2182, 50790, 113976
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NCBI登录号
- NP_004449, NP_075266
抗原
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