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AIRE 抗体 (C-Term)

Name: AIRE 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN184780
Availability: OutOfStock

This anti-AIRE antibody is a 山羊多克隆 antibody detecting AIRE in WB, ELISA, IF, IHC 和 FACS. Suitable for 人.

ABIN184780 (1µg/ml) staining of nuclear Jurkat cell (A), (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). (AIRE 抗体 (C-Term))

ABIN184780 staining (0. (AIRE 抗体 (C-Term))

IHC

ABIN184780 (2µg/ml) staining of paraffin embedded Human Thymus. (AIRE 抗体 (C-Term))

IHC

ABIN184780 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. (AIRE 抗体 (C-Term))

ABIN184780 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (AIRE 抗体 (C-Term))

ABIN184780 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0. (AIRE 抗体 (C-Term))

FACS

8 images

产品编号 ABIN184780

发货至: 中国

Datasheet as PDF

Quick Overview for AIRE 抗体 (C-Term) (ABIN184780)

抗原

See all AIRE 抗体

AIRE (Autoimmune Regulator (AIRE))

适用

人

宿主

山羊

克隆类型

多克隆

标记

This AIRE antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

质量等级

Verified

抗原表位
10

9

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term

原理

AIRE

序列

QSMARPAAPF PS

交叉反应

人

纯化方法

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

免疫原

Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.

亚型

IgG
anti-Autoimmune Regulator (AIRE) (AA 30-130) antibody
AIRE 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (N-Term) antibody
AIRE 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (Internal Region) antibody
AIRE 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (Internal Region) antibody
Verified AIRE 适用: 人, Pig WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (AA 39-108) antibody
AIRE 适用: 人 ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (pSer156) antibody
AIRE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (N-Term) antibody
AIRE 适用: 人, 小鼠, 大鼠, 犬, 马, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (AA 76-125) antibody
AIRE 适用: 人, 马, Cow, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) (AA 467-516) antibody
AIRE 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Autoimmune Regulator (AIRE) antibody
AIRE 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5 μg/mL.
Western Blot: Preliminary testing showed an approx 60 kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room t
Peptide ELISA: antibody detection limit dilution 1:128000.

说明

Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. R

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Minimize freezing and thawing.

储存条件

-20 °C

储存方法

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
抗原

AIRE (Autoimmune Regulator (AIRE))

别名

AIRE

背景

AIRE, autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), APS1, APSI, PGA1, APECED, AIRE protein, autoimmune regulator (APECED protein), autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrop

基因ID

326

NCBI登录号

NP_000374

途径

Chromatin Binding