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NQO1 抗体 (C-Term)

This anti-NQO1 antibody is a 山羊 多克隆 antibody detecting NQO1 in WB, ELISA, IHC 和 IF. Suitable for 人 和 大鼠.
产品编号 ABIN184714
发货至: 中国

Quick Overview for NQO1 抗体 (C-Term) (ABIN184714)

抗原

See all NQO1 抗体
NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

适用

  • 72
  • 34
  • 34
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
人, 大鼠

宿主

  • 68
  • 9
  • 2
  • 1
山羊

克隆类型

  • 59
  • 21
多克隆

标记

  • 48
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This NQO1 antibody is un-conjugated

应用范围

  • 62
  • 26
  • 21
  • 17
  • 17
  • 15
  • 14
  • 14
  • 9
  • 7
  • 4
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 16
    • 8
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    NQO1

    序列

    SIPTDNQIKA RK

    特异性

    This products is expected to recognize all three reported isoforms (NP_000894.1, NP_001020604.1 and NP_001020605.1).

    交叉反应

    犬, 人, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-SIPTDNQIKARK, from the C Terminus of the protein sequence according to NP_000894.1, NP_001020604.1, NP_001020605.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Kidney shows specific staining of the glomeruli. Recommended concentration: 2-4 μg/mL.

    Western Blot: Approx. 30 kDa band observed in lysates of cell lines U251 and HepG2 and in Human Kidney, Lung and Duodenum lysates, and approx. 30-32 kDa band in Rat Kidney, Lung and Duodenum lysates (calculated MW of 30.9 kDa according to Human NP_000894.1

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of HepG2 cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    NQO1 (NAD(P)H Dehydrogenase, Quinone 1 (NQO1))

    别名

    NQO1

    背景

    NQO1, NAD(P)H dehydrogenase, quinone 1, DTD, QR1, DHQU, DIA4, NMOR1, NMORI, diaphorase-4, diaphorase (NADH/NADPH), diaphorase (NADH/NADPH) (cytochrome b-5 reductase), NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1, DT-diaphorase, NAD(P)H menadione

    基因ID

    1728, 18104

    NCBI登录号

    NP_000894, NP_001020604, NP_001020605
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