RACGAP1 抗体 (C-Term)
Quick Overview for RACGAP1 抗体 (C-Term) (ABIN184690)
抗原
See all RACGAP1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- RACGAP1 / MgcRacGAP
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序列
- GRQGNFFASP MLK
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特异性
- This antibody is expected to recognize all reported isoforms (NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_0011
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交叉反应
- Cow, 犬, 人
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1.
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亚型
- IgG
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anti-Rac GTPase Activating Protein 1 (RACGAP1) (AA 1-110) antibody
RACGAP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (N-Term) antibodyRACGAP1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (pSer387) antibodyRACGAP1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (AA 353-632) antibodyRACGAP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (AA 353-632) antibodyRACGAP1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (AA 23-50), (N-Term) antibodyRACGAP1 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB19807 unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (AA 30-58), (N-Term) antibodyRACGAP1 适用: 人 WB, IF, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (pSer387) antibodyRACGAP1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) (AA 86-331) antibodyRACGAP1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Rac GTPase Activating Protein 1 (RACGAP1) antibodyRACGAP1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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Immunohistochemistry: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0 kDa according to NP_037409.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room te
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))
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别名
- RACGAP1
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背景
- RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4
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基因ID
- 29127
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NCBI登录号
- NP_037409, NP_001306934, NP_001306935, NP_001306936
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途径
- Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor
抗原
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