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FOXP3 抗体 (C-Term)

This anti-FOXP3 antibody is a 山羊 多克隆 antibody detecting FOXP3 in WB, FACS, ELISA, IHC, IF 和 ICC. Suitable for 人.
产品编号 ABIN184677
发货至: 中国

Quick Overview for FOXP3 抗体 (C-Term) (ABIN184677)

抗原

See all FOXP3 抗体
FOXP3 (Forkhead Box P3 (FOXP3))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This FOXP3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

质量等级

Verified
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    C-Term

    原理

    FOXP3 / SCURFIN

    序列

    SQRPSRCSNP TPGP

    特异性

    This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence SQRPSRCSNPTPGP, from the C Terminus of the protein sequence according to NP_054728.2, NP_001107849.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

    Western Blot: Approx 48-50 kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2 kDa according to NP_054728.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negat

    Peptide ELISA: antibody detection limit dilution 1:8000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    FOXP3 (Forkhead Box P3 (FOXP3))

    别名

    FOXP3

    背景

    FOXP3, SCURFIN, forkhead box P3, JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2 protein, immunodeficiency, polyendocrinopathy, enteropathy, X-linked, immune dysregulation, polyendocrinopathy, enteropathy, X-linked, MGC141961, MGC141963, FOXP3delta7, scurfin

    基因ID

    50943

    NCBI登录号

    NP_054728, NP_001107849

    途径

    Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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