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NCF1 抗体 (C-Term)

This anti-NCF1 antibody is a 山羊 多克隆 antibody detecting NCF1 in WB, ELISA, IHC, IF 和 FACS. Suitable for 人, 小鼠 和 Pig.
产品编号 ABIN184583
发货至: 中国

Quick Overview for NCF1 抗体 (C-Term) (ABIN184583)

抗原

See all NCF1 抗体
NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

适用

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人, 小鼠, Pig

宿主

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山羊

克隆类型

  • 123
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多克隆

标记

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This NCF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    NCF1 / p47phox

    序列

    SESTKRKLAS AV

    交叉反应

    Cow, 人, 小鼠, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45

    Peptide ELISA: antibody detection limit dilution 1:20000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

    别名

    NCF1

    背景

    NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2

    基因ID

    653361, 17969, 114553

    NCBI登录号

    NP_000256

    途径

    PI3K-Akt Signaling
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