Strep Tag II 抗体 (HRP)
This anti- antibody (ABIN1842500) is a Rabbit Polyclonal antibody detecting in WB, ELISA. Suitable for .
Quick Overview for Strep Tag II 抗体 (HRP) (ABIN1842500)
抗原
Strep Tag II
适用
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宿主
兔
克隆类型
多克隆
标记
HRP
应用范围
Western Blotting (WB), ELISA
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序列
- NWSHPQFEK
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特异性
- This Antibody recognizes Strep-tag II tagged fusion proteins.
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纯化方法
- Immunoaffinity
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免疫原
- Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH.
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亚型
- IgG
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anti-Strep Tag II antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Strep Tag II antibody (Biotin)WB, ELISA 宿主: 小鼠 Monoclonal 5A9F9 Biotin
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0. 05-0. 2 µg/mL
Western blot: 0. 5-1. 0 µg/mL -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
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浓度
- 0.5 mg/mL
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缓冲液
- lyophilized with PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
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- Strep Tag II
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物质类
- Tag
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背景
- Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK.
抗原
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