Strep Tag II 抗体
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- 抗原
- Strep Tag II
- 适用
- Various Species
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 纯化方法
- Protein A/G Purified
- 免疫原
- Synthetic peptide corresponding to Strep II tag conjugated to keyhole limpet haemocyanin.
- 克隆位点
- 4H10
- 亚型
- IgG1
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anti-Strep Tag II antibody
WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Strep Tag II antibodyWB 宿主: 小鼠 Monoclonal 1L2 unconjugated
anti-Strep Tag II antibody (Biotin)WB, ELISA 宿主: 小鼠 Monoclonal 5A9F9 Biotin
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- 应用备注
- WB: 1:1000-2000, IP: 3 μg/sample, IF: 1:100-200
- 限制
- 仅限研究用
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- 浓度
- 4.5 mg/mL
- 缓冲液
- PBS with 0.1 % sodium azide, 50 % glycerol, pH 7.4
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Avoid freeze / thaw cycles.
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- 抗原
- Strep Tag II
- 别名
- Strep-Tag II
- 物质类
- Tag
- 背景
- Strep Tag II, Strep II,The Strep-tag II is a synthetic peptide consisting of eight amino acids ( WSHPQFEK ). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant protein. The Strep-tag II peptide was developed as an affinity tool for the purification of corresponding fusion proteins on streptavidin columns. The peptide recognizes the same pocket of streptavidin where the natural ligand is normally bound so that biotin or its chemical derivatives can be used for competitive elution.
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