CD8 抗体 (FITC)
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北京 101111
Quick Overview for CD8 抗体 (FITC) (ABIN182025)
抗原
See all CD8 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody, clone YCATE 55.9 was clustered as CD8 in the first canine leukocyte antigen workshop. The antibody reacts with canine CD8a transfectants. Clone YCATE 55.9 is reported to block MHC class I dependant T-cell responses in vitro and in vivo.
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产品特性
- Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Canine CD8a chimaeric human IgG1 Fc fusion protein. Spleen cells from immunised DA ratwere fused with cells of the Y3/A1. 2. 3 rat myeloma cell line.
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亚型
- IgG1
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anti-CD8 (CD8) (AA 51-150) antibody
CD8 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibody (PE)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE
anti-CD8 (CD8) (AA 22-187) antibodyCD8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) (AA 135-235) antibodyCD8 适用: 人 IHC 宿主: 兔 Monoclonal 008R unconjugated Recombinant Antibody
anti-CD8 (CD8) antibodyCD8 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-31 unconjugated
anti-CD8 (CD8) antibody (Biotin)CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin
anti-CD8 (CD8) antibody (FITC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC
anti-CD8 (CD8) antibody (PE)CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE
anti-CD8 (CD8) antibody (APC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC
anti-CD8 (CD8) antibody (PerCP)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP
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应用备注
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Flow Cytometry: Neat - 1/5, use 10 μL of the suggested working dilution to label 10^6 cells in100l.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: one year from despatch. -
有效期
- 12 months
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- CD8
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别名
- CD8
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背景
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
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基因ID
- 403157
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UniProt
- P33706
抗原
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