Betacellulin 抗体 (Biotin)
Quick Overview for Betacellulin 抗体 (Biotin) (ABIN181705)
抗原
See all Betacellulin (BTC) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Betacellulin.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human Betacellulin
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应用备注
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ELISA: Direct: To detect hBetacellulin (using 100 μL/well antibody solution) a concentrationof 0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin. Sandwich: To detect hBetacellulin (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanBetacellulin as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hBetacellulin. Western blot: To detect hBetacellulin this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- Betacellulin (BTC)
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别名
- Betacellulin
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背景
- Betacellulin is a member of the mammalian members of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This encoded protein is a ligand for the EGF receptor. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.Synonyms: BTC, Probetacellulin
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基因ID
- 685
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NCBI登录号
- NP_001720
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UniProt
- P35070
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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