CTGF
适用: 人
WB, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: Direct: To detect hCTGF (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF. Sandwich: To detect hCTGF (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanCTGF as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCTGF. Western blot: To detect hCTGF this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.2 without preservatives
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
The lyophilized antibody is stable for at least 2 years from date of receipt at -20 °C. Store the reconstituted antibody at 2-8 °C for one month or at -20 °C (in aliquots) for longer.
CTGF (connective tissue growth factor) is a 38 kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.Synonyms: CCN family member 2, CCN2, Connective tissue growth factor, HCS24, Hypertrophic chondrocyte-specific protein 24, IBP8, IGF-binding protein 8, IGFBP8, Insulin-like growth factor-binding protein 8