ELISA: Indirect: To detect hCTGFL/WISP-2 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCTGFL/WISP-2. Sandwich: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human CTGFL/WISP-2 as a detection antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
缓冲液
PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
有效期
6 months
抗原
WISP2
(WNT1 Inducible Signaling Pathway Protein 2 (WISP2))