WISP2 抗体 (Biotin)
Quick Overview for WISP2 抗体 (Biotin) (ABIN181621)
抗原
See all WISP2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Connective Tissue Growth Factor-Like protein.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human CTGFL/WISP-2
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应用备注
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ELISA: Direct: To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibodysolution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunctionwith compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCTGFL/WISP-2. Sandwich: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunctionwith Polyclonal Anti-Human CTGFL/WISP-2 as a capture antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))
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别名
- WISP2
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背景
- Synonyms: CCN5, CT58, CTGF-L, CTGFL, Connective tissue growth factor-like protein, Connective tissue growth factor-related protein 58, UNQ228/PRO261, WISP-2, WNT1-inducible-signaling pathway protein 2
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基因ID
- 8839
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NCBI登录号
- NP_003872
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UniProt
- O76076
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途径
- WNT signaling, Growth Factor Binding
抗原
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