CLCF1 抗体
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Quick Overview for CLCF1 抗体 (ABIN181612)
抗原
See all CLCF1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects NNT-1/BCSF-3.
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纯化方法
- Immunoaffinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human NNT-1/BCSF-3
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anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) (AA 88-190) antibody
CLCF1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) (AA 28-225) antibodyCLCF1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) antibodyCLCF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) antibodyCLCF1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) (AA 29-225) antibodyCLCF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7E10 unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) (AA 1-225) antibodyCLCF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) (AA 28-178) antibodyCLCF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cardiotrophin-Like Cytokine Factor 1 (CLCF1) (AA 46-72), (N-Term) antibodyCLCF1 适用: 人 WB 宿主: 兔 Polyclonal RB38733 unconjugated
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应用备注
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ELISA: Indirect: To detect hNNT-1/BCSF-3 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hNNT-1/BCSF-3. Sandwich: To detect hNNT-1/BCSF-3 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withBiotinylated Anti-Human NNT-1/BCSF-3 as a detection antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hNNT-1/BCSF-3. Western Blot: To detect hNNT-1/BCSF-3 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hNNT-1/BCSF-3 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- CLCF1 (Cardiotrophin-Like Cytokine Factor 1 (CLCF1))
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别名
- CLCF1
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背景
- NNT1 belongs to the interleukin 6 family of cytokines, which are involved in cell signaling through phosphorylation of gp130. IL6 family members share similarity in gene structure and have a 4 helix bundle in their protein structure. NNT1 has a sequence of 225-amino acids with a 27-aa signal peptide, a molecular mass of 22 kDa in mature form, and the highest homology to cardiotrophin-1 and ciliary neurotrophic factor. NNT1 may act as a neuroimmunoendocrine modulator of hypothalamus-pituitary-adrenal axis stress response.Synonyms: B-cell-stimulating factor 3, BSF3, CLC, Cardiotrophin-like cytokine factor 1, NNT1, Novel neurotrophin-1
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基因ID
- 23529
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NCBI登录号
- NP_037378
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UniProt
- Q9UBD9
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
抗原
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