BMP7 抗体 (Biotin)
Quick Overview for BMP7 抗体 (Biotin) (ABIN181549)
抗原
See all BMP7 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects BMP7.
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纯化方法
- Affinity chromatography
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免疫原
- Highly pure (> 98 %) recombinant human BMP-7
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anti-Bone Morphogenetic Protein 7 (BMP7) (AA 1-431) antibody
BMP7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal M1-F8 unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 132-431) antibodyBMP7 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 239-431) antibodyBMP7 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6E5D12 unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (N-Term) antibodyBMP7 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, Pig, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 293-350) antibodyBMP7 适用: 小鼠, 大鼠, 犬, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 293-431) antibodyBMP7 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (N-Term) antibodyBMP7 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 绵羊, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 15-44) antibodyBMP7 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 280-309) antibodyBMP7 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bone Morphogenetic Protein 7 (BMP7) (AA 123-393) antibodyBMP7 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: Direct: To detect BMP-7 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant BMP-7. Sandwich: To detect BMP-7 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human BMP-7 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant BMP-7. Western Blot: To detect BMP-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant BMP-7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
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别名
- BMP7
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背景
- Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1
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基因ID
- 655
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NCBI登录号
- NP_001710
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UniProt
- P18075
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途径
- Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
抗原
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