WNT3A 抗体 (Biotin)
Quick Overview for WNT3A 抗体 (Biotin) (ABIN181373)
抗原
See all WNT3A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects Human WNT-3a. Other species not tested.
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纯化方法
- Affinity Chromatography
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免疫原
- E.Coli derived recombinant Human WNT-3a
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anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 281-352) antibody
WNT3A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-290) antibodyWNT3A 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-290) antibodyWNT3A 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-352) antibodyWNT3A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-385) antibodyWNT3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (Internal Region) antibodyWNT3A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 227-276) antibodyWNT3A 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (N-Term) antibodyWNT3A 适用: 人, 小鼠, 大鼠, 兔, 豚鼠, 斑马鱼, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 1-385) antibodyWNT3A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) antibodyWNT3A 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3A6 unconjugated
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应用备注
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ELISA: Direct: To detect hWNT-3a by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. This biotinylated polyclonal antibody, inconjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Sandwich: To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. This biotinylated polyclonal antibody, inconjunction with Polyclonal Anti-Human WNT-3a as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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缓冲液
- PBS, pH 7.2
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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有效期
- 6 months
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- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
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别名
- WNT3A
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背景
- Synonyms: Protein Wnt-3a
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基因ID
- 89780
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NCBI登录号
- NP_149122
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UniProt
- P56704
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途径
- WNT signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
抗原
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