Nerve Growth Factor 抗体
Quick Overview for Nerve Growth Factor 抗体 (ABIN181153)
抗原
See all Nerve Growth Factor (NGF) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects NGF-beta.
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产品特性
- Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor
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纯化方法
- Affinity Chromatography on Protein G
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免疫原
- Highly pure (>98%) recombinant Human beta-NGF
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亚型
- IgG2b
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anti-Nerve Growth Factor (NGF) antibody
NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 19-241) antibodyNGF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 162-173), (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-218) antibodyNGF 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) (AA 122-241) antibodyNGF 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibodyNGF 适用: 人 WB, IHC, ELISA 宿主: 兔 Monoclonal 5F10 unconjugated Recombinant Antibody
anti-Nerve Growth Factor (NGF) (N-Term) antibodyNGF 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor (NGF) antibody (Biotin)NGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal Biotin
anti-Nerve Growth Factor (NGF) (AA 19-241) antibodyNGF 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman beta-NGF (10.0 ng/mL), a concentration of 2.7-4.0 μg/mL of this antibody isrequired. ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 200 pg/mL of recombinant Human Beta-NGF when used withBiotin anti-Human beta-NGF antibody (cat. PP1057B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can beused at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human beta-NGF is 4.0-8.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
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缓冲液
- PBS without preservatives
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
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The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Nerve Growth Factor (NGF)
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别名
- NGF
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背景
- Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB
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基因ID
- 4803
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UniProt
- P01138
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途径
- Regulation of Cell Size
抗原
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