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tau 抗体 (N-Term)

Cited in 2+ publications. This 小鼠 单克隆 anti-tau antibody (Clone 248E5) specifically detects tau in WB, IHC, IHC (p), ICC 和 IP. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN1742567
发货至: 中国
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Quick Overview for tau 抗体 (N-Term) (ABIN1742567)

抗原

See all tau 抗体
tau (tau Protein)

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This tau antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

克隆位点

248E5
  • 抗原表位

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    N-Term

    原理

    Mouse anti-Tau IgG

    特异性

    Specific for tau.

    交叉反应 (详细)

    Reacts with: rat (P19332), mouse (P10637). Weaker signal: human (P10636). No signal: zebrafish. Other species not tested yet.

    纯化方法

    purified IgG

    免疫原

    Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)

    亚型

    IgG2a
  • 应用备注

    WB:1 : 1000 , IP:yes ICC:1 : 200 up to 1 : 500, IHC:1 : 100, IHC-P:1 : 500,

    说明

    WB: Detects the mouse protein with much greater sensitivity than the rat protein. The antibody binds phosphorylated and non-phosphorylated tau proteins.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

    缓冲液

    PBS. Albumin and azide were added for stabilization.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    储存条件

    -20 °C,-80 °C,4 °C

    储存方法

    Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!
  • Gut, Beske, Hubbard, Lyman, Hamilton, McNutt: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, (2013) (PubMed).

    Ahmad, Wolber, Eckardt, Koch, Schmitt, Semechkin, Geis, Heckmann, Brüstle, McLaughlin, Sirén, Müller: "Functional neuronal cells generated by human parthenogenetic stem cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e42800, (2012) (PubMed).

  • 抗原

    tau (tau Protein)

    别名

    Tau

    背景

    Synonyms: Microtubule-associated protein tau, Mapt, MAPT

    Antigen Information: Tau is a neuronal microtubule associated protein

    UniProt

    P10637
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