Abeta 1-42 抗体 (AA 37-42)
(6 references)Quick Overview for Abeta 1-42 抗体 (AA 37-42) (ABIN1742444)
抗原
See all Abeta 1-42 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 37-42
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特异性
- Specific for Abeta 42
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交叉反应 （详细）
- weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.
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纯化方法
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
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免疫原
- Synthetic peptide corresponding to aa 37-42 of Abeta peptide 42 coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
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anti-Amyloid beta 1-42 (Abeta 1-42) antibody
Abeta 1-42 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Amyloid beta 1-42 (Abeta 1-42) (AA 1-42) antibodyAbeta 1-42 适用: 人, 大鼠, 小鼠 WB, ICC, IF (p), ELISA, FACS, IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta 1-42 (Abeta 1-42) antibodyAbeta 1-42 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 295F2 unconjugated
anti-Amyloid beta 1-42 (Abeta 1-42) antibodyAbeta 1-42 适用: 人 WB, ELISA, DB 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta 1-42 (Abeta 1-42) antibodyAbeta 1-42 适用: 人 WB, ELISA, DB, IL 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1 : 1000 (ECL detection)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500
IHC-P: 1 : 100
ELISA: yes, suitable only as capture antibody, ABIN1742439 is recommended detector antibody for sandwich-ELISA (protocol) -
说明
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WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
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缓冲液
- PBS
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注意事项
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
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储存条件
- -20 °C
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储存方法
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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: "Significance of cytosolic cathepsin D in Alzheimer's disease pathology: Protective cellular effects of PLGA nanoparticles against β-amyloid-toxicity." in: Neuropathology and applied neurobiology, Vol. 46, Issue 7, pp. 686-706, (2021) (PubMed).
: "Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 44, Issue 4, pp. 1291-302, (2015) (PubMed).
: "Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease." in: Molecular neurodegeneration, Vol. 9, pp. 13, (2014) (PubMed).
: "Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 4, pp. 871-81, (2014) (PubMed).
: "Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal A? accumulation." in: Frontiers in aging neuroscience, Vol. 6, pp. 139, (2014) (PubMed).
: "N-truncated amyloid β (Aβ) 4-42 forms stable aggregates and induces acute and long-lasting behavioral deficits." in: Acta neuropathologica, Vol. 126, Issue 2, pp. 189-205, (2013) (PubMed).
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: "Significance of cytosolic cathepsin D in Alzheimer's disease pathology: Protective cellular effects of PLGA nanoparticles against β-amyloid-toxicity." in: Neuropathology and applied neurobiology, Vol. 46, Issue 7, pp. 686-706, (2021) (PubMed).
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- Abeta 1-42 (Amyloid beta 1-42 (Abeta 1-42))
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别名
- Abeta 42
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背景
- Synonyms: Beta-App, ABPP
抗原
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