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TNC 抗体

This anti-TNC antibody is a 大鼠 单克隆 antibody detecting TNC in WB, IHC 和 ICC. Suitable for 大鼠 和 小鼠. This Primary Antibody has been cited in 5+ publications.
产品编号 ABIN1742432
发货至: 中国

Quick Overview for TNC 抗体 (ABIN1742432)

抗原

See all TNC 抗体
TNC (Tenascin C (TNC))

适用

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大鼠, 小鼠

宿主

  • 97
  • 54
  • 2
  • 1
  • 1
大鼠

克隆类型

  • 81
  • 74
单克隆

标记

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  • 3
  • 3
  • 3
  • 3
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  • 3
  • 2
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  • 2
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  • 2
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This TNC antibody is un-conjugated

应用范围

  • 92
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  • 12
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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

克隆位点

578
  • 特异性

    Specific for tenascin-C splice variants carrying the FNIII D domain.

    纯化方法

    hybridoma supernatant

    免疫原

    Glycopeptidase F-treated tenascin from mouse brain. Epitope FNIII D domain

    亚型

    IgG
  • 应用备注

    1:10.000 recommended dilution

    说明

    Tenascin-C variants detected by this antibody are downregulated during development and hardly detectable in adult animals

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.

    缓冲液

    PBS

    注意事项

    When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
    Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.

    储存条件

    -20 °C

    储存方法

    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Siddiqui, Horvat-Bröcker, Faissner: "The glia-derived extracellular matrix glycoprotein tenascin-C promotes embryonic and postnatal retina axon outgrowth via the alternatively spliced fibronectin type III domain TNfnD." in: Neuron glia biology, Vol. 4, Issue 4, pp. 271-83, (2010) (PubMed).

    Rigato, Garwood, Calco, Heck, Faivre-Sarrailh, Faissner: "Tenascin-C promotes neurite outgrowth of embryonic hippocampal neurons through the alternatively spliced fibronectin type III BD domains via activation of the cell adhesion molecule F3/contactin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 15, pp. 6596-609, (2002) (PubMed).

    Bartsch, Bartsch, Dörries, Faissner, Weller, Ekblom, Schachner: "Expression of tenascin in the developing and adult cerebellar cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 3, pp. 736-49, (1992) (PubMed).

    Stern, Norris, Bronner-Fraser, Carlson, Faissner, Keynes, Schachner: "J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo." in: Development (Cambridge, England), Vol. 107, Issue 2, pp. 309-19, (1990) (PubMed).

    Gatchalian, Schachner, Sanes: "Fibroblasts that proliferate near denervated synaptic sites in skeletal muscle synthesize the adhesive molecules tenascin(J1), N-CAM, fibronectin, and a heparan sulfate proteoglycan." in: The Journal of cell biology, Vol. 108, Issue 5, pp. 1873-90, (1989) (PubMed).

  • 抗原

    TNC (Tenascin C (TNC))

    别名

    Tenascin-C

    背景

    Synonyms: TN-C, J1-200/220, Cytoactin

    途径

    Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
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