GAD65 抗体 (N-Term)
(3 references)Quick Overview for GAD65 抗体 (N-Term) (ABIN1742417)
抗原
See all GAD65 (GAD2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Mouse anti-GAD2 IgG
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特异性
- Specific for GAD 2 / GAD 65
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交叉反应 （详细）
- Reacts with: rat (Q05683), mouse (P48320), human (Q05329). Other species not tested yet.
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纯化方法
- purified IgG
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免疫原
- Recombinant protein corresponding to the amino terminus of mouse GAD2 (UniProt Id: P48320) Epitop AA 3 to 17 from mouse GAD2 (UniProt Id: P48320)
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亚型
- IgG3
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anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 65-105) antibody
GAD2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) antibodyGAD2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 188-374) antibodyGAD2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 401-585) antibodyGAD2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (Internal Region) antibodyVerified GAD2 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (C-Term) antibodyGAD2 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-100) antibodyGAD2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5D9G12 unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 131-164), (N-Term) antibodyGAD2 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 109-138) antibodyGAD2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB19330 unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 501-585) antibodyGAD2 适用: 人, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB:1 : 1000 , IP:yes ICC:1 : 500, IHC:1 : 200 up to 1 : 500, IHC-P:1 : 100 up to 1 : 1000, ELISA:yes
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说明
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WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.
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缓冲液
- PBS. Albumin and azide were added for stabilization.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
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储存条件
- -20 °C,-80 °C,4 °C
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储存方法
- Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!
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: "Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).
: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, (2014) (PubMed).
: "Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons." in: Neuroscience, Vol. 246, pp. 451-67, (2013) (PubMed).
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: "Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." in: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).
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- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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别名
- GAD2
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背景
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Synonyms: Glutamic acid decarboxylase, GAD65, Glutamic acid decarboxylase 2, Glutamic acid decarboxylase 65
Antigen Information: GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter
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UniProt
- P48320
抗原
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