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Bassoon 抗体 (C-Term)

Name: Bassoon 抗体 (C-Term)
SKU: ABIN1742310
Availability: OutOfStock

BSN 适用: 大鼠, 小鼠 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 219E1 unconjugated

$dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2) (Bassoon 抗体 (C-Term))$ WB

Immunocytochemistry on cultured hippocampus neurons (dilution 1 : 300). (Bassoon 抗体 (C-Term))

ICC

2 images

(7 references)

产品编号 ABIN1742310

发货至: 中国

Datasheet as PDF

抗原 See all Bassoon (BSN) 抗体

Bassoon (BSN)
抗原表位
13

2

1

1

1
C-Term
适用
25

14

13

1

1
大鼠, 小鼠
宿主
26

2
小鼠
Compatible Secondaries
克隆类型
26

2
单克隆
标记
12

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Bassoon antibody is un-conjugated
应用范围
19

18

4

3

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

特异性

Specific for bassoon.

纯化方法

hybridoma supernatant

免疫原

Recombinant protein containing the 330 C-terminal aa of rat bassoon.

克隆位点

219E1

亚型

IgG2b

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BSN 适用: 大鼠, 小鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated

anti-Bassoon (BSN) antibody
BSN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Bassoon (BSN) antibody
BSN 适用: 人, 大鼠, 小鼠, Cow, 犬 IHC, WB, IF, ICC, IP 宿主: 小鼠 Monoclonal SAP7F407 unconjugated

anti-Bassoon (BSN) (AA 786-1041) antibody
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated

anti-Bassoon (BSN) (AA 786-1041) antibody (HRP)
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal HRP

anti-Bassoon (BSN) (AA 786-1041) antibody (FITC)
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal FITC

anti-Bassoon (BSN) (AA 786-1041) antibody (Biotin)
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal Biotin

anti-Bassoon (BSN) (AA 786-1041) antibody (PE)
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal PE

anti-Bassoon (BSN) (AA 786-1041) antibody (APC)
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal APC

anti-Bassoon (BSN) (AA 786-1041) antibody (PerCP)
BSN 适用: 人 IHC, WB 宿主: 兔 Polyclonal PerCP

应用备注

WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 500
IHC: not tested yet

说明

ICC: Mild fixation conditions (2 % PFA for 20 min) are recommended. ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

限制

仅限研究用
状态

Liquid

缓冲液

PBS

注意事项

When hybridoma supernatant is reconstituted, we usually add small amounts of azide or thimerosal to prevent microbial growth. Ascites should be stored frozen (-20 °C). Monoclonals usually do not suffer from repetitive freeze-thawing but you may aliquot them into small samples to avoid too many freeze-thaw cycles.
Prolonged storage at 4 °C is not recommended! Unlike serum, ascites may contain proteases that will ultimately degrade the antibodies. Addition of protease inhibitors helps to slow degradation.

储存条件

-20 °C

储存方法

Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Schafer, Heller, Gunner, Heller, Gordon, Hammond, Wolf, Jung, Stevens: "Microglia contribute to circuit defects in Mecp2 null mice independent of microglia-specific loss of Mecp2 expression." in: eLife, Vol. 5, (2016) (PubMed).

Hagmeyer, Mangus, Boeckers, Grabrucker: "Effects of trace metal profiles characteristic for autism on synapses in cultured neurons." in: Neural plasticity, Vol. 2015, pp. 985083, (2015) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Schröder, Stellmacher, Romorini, Marini, Montenegro-Venegas, Altrock, Gundelfinger, Fejtova: "Regulation of presynaptic anchoring of the scaffold protein Bassoon by phosphorylation-dependent interaction with 14-3-3 adaptor proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58814, (2013) (PubMed).

Orenbuch, Shalev, Marra, Sinai, Lavy, Kahn, Burden, Staras, Gitler: "Synapsin selectively controls the mobility of resting pool vesicles at hippocampal terminals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 12, pp. 3969-80, (2012) (PubMed).

Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

Morgans, Zhang, Jeffrey, Nelson, Burke, Duvoisin, Brown: "TRPM1 is required for the depolarizing light response in retinal ON-bipolar cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 45, pp. 19174-8, (2009) (PubMed).
抗原

Bassoon (BSN)

别名

Bassoon (BSN 产品)

别名

Znf231 antibody, ZNF231 antibody, bassoon antibody, bassoon (presynaptic cytomatrix protein) antibody, bassoon presynaptic cytomatrix protein antibody, Bsn antibody, BSN antibody