Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
特异性
Reacts withepitopes common to many human amyloid fibrils and fibrillar oligomers but not prefibrillar oligomers or natively folded proteins. Predicted to recognize mouse and rat based on sequence homology.
Working dilution: Optimal dilutions should be determined by the end user. The following are guidelines only: WB1 -2 μg/mL DB0.5 - 1 μg/mL IHC1 -5 μg/mL ELISA1 - 10 μg/mL with amyloid fibril-containing samples on the solid phase.
限制
仅限研究用
状态
Liquid
浓度
0.5 mg/mL
缓冲液
PBS pH 7.0, 50 % glycerol
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute in PBS or medium which is identical to that used in the assay system.
储存条件
-20 °C
储存方法
This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
抗原
Amyloid Fibrils
背景
Amyloid monomeric proteins can oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibers.