AGO2 抗体
Quick Overview for AGO2 抗体 (ABIN1720882)
抗原
See all AGO2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- TAIR: At1g48410
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交叉反应 (详细)
- Not reactive in: Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays
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预测反应
- Capsella rubella, Solanum tuberosum
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纯化方法
- serum
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免疫原
- KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280
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应用备注
- 1: 250 - 1: 500 (WB)
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说明
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AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water.
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注意事项
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Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. -
储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- AGO2 (Argonaute 2 (AGO2))
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别名
- AGO2
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背景
- AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
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分子量
- expected: 116.4 kDa, appearent: 130 kDa
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UniProt
- Q9SHF3
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
抗原
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