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AGO2 抗体

This 兔 多克隆 antibody specifically detects AGO2 in WB 和 IP. It exhibits reactivity toward Arabidopsis thaliana.
产品编号 ABIN1720882
发货至: 中国
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Quick Overview for AGO2 抗体 (ABIN1720882)

抗原

See all AGO2 抗体
AGO2 (Argonaute 2 (AGO2))

适用

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Arabidopsis thaliana

宿主

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克隆类型

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多克隆

标记

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This AGO2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP)
  • 原理

    Anti-AGO2 | Argonaute 2

    特异性

    TAIR: At1g48410

    交叉反应 (详细)

    Arabidopsis thaliana
    Not reactive in: Chlamydomonas reinhardtii, Medicago truncatula, Nicotiana benthamiana, Populus sp., Solanum lycopersicum, Zea mays

    预测反应

    Capsella rubella,Solanum tuberosum

    纯化方法

    Immunogen affinity purified

    免疫原

    KLH-conjugated synthetic peptide
  • 应用备注

    1 : 250-1 : 500 (WB)

    说明

    AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μL of sterile water

    缓冲液

    Immunogen affinity purified IgG in PBS pH 7.4.

    注意事项

    Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

    储存条件

    -20 °C

    储存方法

    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
  • 抗原

    AGO2 (Argonaute 2 (AGO2))

    别名

    AGO2

    背景

    AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.

    分子量

    113 kDa

    基因ID

    840016

    UniProt

    Q9SHF3

    途径

    Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
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