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EIF2A 抗体 (pSer52)

Name: EIF2A 抗体 (pSer52)
SKU: ABIN1714407
Availability: OutOfStock

This anti-EIF2A antibody is a 兔多克隆 antibody detecting EIF2A in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

Paraformaldehyde-fixed, paraffin embedded rat lung tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-eIF2 alpha (Ser51) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (EIF2A 抗体 (pSer52))

IHC (p)

1 image

(3 references)

产品编号 ABIN1714407

发货至: 中国

Datasheet as PDF

Quick Overview for EIF2A 抗体 (pSer52) (ABIN1714407)

抗原

See all EIF2A 抗体

EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This EIF2A antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Discover our top product EIF2A Primary Antibody

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pSer52

交叉反应

人, 小鼠, 大鼠

预测反应

Cow

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from human eIF2 alpha subunit 1 around the phosphorylation site of Ser52 [EL(p-S)RR]

亚型

IgG
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 448-576) antibody
EIF2A 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3A7A8 unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 448-576) antibody
EIF2A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A7B11 unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibody
EIF2A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 437-465) antibody
EIF2A 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibody
EIF2A 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 1-100) antibody
EIF2A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D5 unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (Center) antibody
EIF2A 适用: 人, 小鼠, 大鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (pSer51) antibody
EIF2A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (Ser326) antibody
EIF2A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 504-553) antibody
EIF2A 适用: 人, 猴, 犬, 马, Cow, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Cha, Song, Kang, Park, Park, Kim, Shim, Kim: "miR-211 Plays a Critical Role in Cnidium officinale Makino Extract-Induced, ROS/ER Stress-Mediated Apoptosis in U937 and U266 Cells." in: International journal of molecular sciences, Vol. 19, Issue 3, (2018) (PubMed).

Yao, Lu, Hu, Yu, Chen, Wang: "A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure." in: Nature communications, Vol. 8, Issue 1, pp. 133, (2017) (PubMed).

He, Long, Zhong, Fu, Li, Lin: "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." in: Molecular and cellular biochemistry, Vol. 389, Issue 1-2, pp. 151-8, (2014) (PubMed).
抗原

EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

别名

eIF2 alpha

背景

Synonyms: EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, EIF2S1
Background: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 4S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 6S ribosomal subunit to form the 8S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

基因ID

1965

UniProt

P05198

途径

Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly