ZBTB7A 抗体 (AA 321-420)
Quick Overview for ZBTB7A 抗体 (AA 321-420) (ABIN1714221)
抗原
See all ZBTB7A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 321-420
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交叉反应
- 人
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预测反应
- Mouse,Rat,Dog,Cow,Sheep,Pig,Chicken
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纯化方法
- Purified by Protein A.
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免疫原
- KLH conjugated synthetic peptide derived from human Pokemon
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亚型
- IgG
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anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (AA 151-250) antibody
ZBTB7A 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) antibodyZBTB7A 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (N-Term) antibodyZBTB7A 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (N-Term) antibodyZBTB7A 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (AA 1-108) antibodyZBTB7A 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (C-Term) antibodyZBTB7A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (AA 125-163), (N-Term) antibodyZBTB7A 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (AA 224-273) antibodyZBTB7A 适用: 人, 小鼠, 大鼠, 犬, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (AA 114-163) antibodyZBTB7A 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Zinc Finger and BTB Domain Containing 7A (ZBTB7A) (AA 108-157) antibodyZBTB7A 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, Pig, 猴, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- ZBTB7A (Zinc Finger and BTB Domain Containing 7A (ZBTB7A))
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别名
- Pokemon/ZBTB7
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背景
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Synonyms: Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, FBI1, HIV-1 1st-binding protein 1, Leukemia/lymphoma related factor, LRF, Pokemon, TIP21, TTF-I interacting peptide 21, ZBTB7, ZBTB7A, Zinc finger and BTB domain-containing protein 7A, ZBT7A_HUMAN.
Background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy.
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基因ID
- 51341
抗原
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