我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

FZD3 抗体 (AA 121-220)

Name: FZD3 抗体 (AA 121-220)
SKU: ABIN1714171
Availability: OutOfStock

This anti-FZD3 antibody is a 兔多克隆 antibody detecting FZD3 in WB, ELISA, IHC (p) 和 IHC (fro). Suitable for 人, 小鼠, 大鼠和非洲爪蟾. This Primary Antibody has been cited in 1 publication.

Lane 1: mouse pancreas lysate probed with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated (ABIN1714171) at 1:300 overnight at 4 °C. (FZD3 抗体 (AA 121-220))

Paraformaldehyde-fixed, paraffin embedded human colon cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated ( ) at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated-Cy)and used at 1:200 dilution for 40 minutes at 37°CDAPI(5ug/ml) was used to stain the cell nuclei (FZD3 抗体 (AA 121-220))

IF (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by the secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated-Cy3)used at 1:200 dilution for 40 minutes at 37°C. (FZD3 抗体 (AA 121-220))

IF (p)

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FZD3/Frizzled 3 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (FZD3 抗体 (AA 121-220))

IHC (p)

4 images

(1 reference)

产品编号 ABIN1714171

发货至: 中国

Quick Overview for FZD3 抗体 (AA 121-220) (ABIN1714171)

抗原

See all FZD3 抗体

FZD3 (Frizzled Family Receptor 3 (FZD3))

适用

人, 小鼠, 大鼠, 非洲爪蟾

宿主

兔

克隆类型

多克隆

标记

This FZD3 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
8

5

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 121-220

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3

亚型

IgG
anti-Frizzled Family Receptor 3 (FZD3) (AA 23-205) antibody
FZD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibody
FZD3 适用: 人, 小鼠, 大鼠, Bat, Cow, 犬, Hamster, 马, 猴, Pig, 兔, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibody
FZD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2H5 unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibody
FZD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B8 unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibody
FZD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibody
FZD3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibody
FZD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 581-596), (Intracellular) antibody
FZD3 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (AA 481-530) antibody
FZD3 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibody
FZD3 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Su, Zhang, Gan, Jiang, Xiao, Zou, Li, Jiang: "Deregulation of the planar cell polarity genes CELSR3 and FZD3 in Hirschsprung disease." in: Experimental and molecular pathology, Vol. 101, Issue 2, pp. 241-248, (2016) (PubMed).
抗原

FZD3 (Frizzled Family Receptor 3 (FZD3))

别名

FZD3/Frizzled 3

背景

Synonyms: Fz-3, Frizzled-3, hFz3, FZD3
Background: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

基因ID

7976

UniProt

Q9NPG1

途径

WNT signaling, Tube Formation