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FFAR2 抗体 (AA 41-140)

Name: FFAR2 抗体 (AA 41-140)
SKU: ABIN1713521
Availability: OutOfStock

This anti-FFAR2 antibody is a 兔多克隆 antibody detecting FFAR2 in WB, ELISA, IHC (p), IF (p), FACS 和 IHC (fro). Suitable for 小鼠和大鼠. This Primary Antibody has been cited in 1 publication.

Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-GPR43 Polyclonal Antibody, Unconjugated (ABIN1713521) at 1:200 followed by conjugation to the secondary antibody and DAB staining (FFAR2 抗体 (AA 41-140))

IHC

Mouse splenocytes probed with Rabbit Anti-GPR43 Polyclonal Antibody, Unconjugated . (FFAR2 抗体 (AA 41-140))

FACS

2 images

(1 reference)

产品编号 ABIN1713521

发货至: 中国

Quick Overview for FFAR2 抗体 (AA 41-140) (ABIN1713521)

抗原

See all FFAR2 抗体

FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

适用

小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This FFAR2 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

抗原表位
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1
AA 41-140

交叉反应

小鼠, 大鼠

预测反应

Human

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human GPR43

亚型

IgG
anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 301-314), (Intracellular) antibody
FFAR2 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 277-330) antibody
FFAR2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (Cytoplasmic Domain) antibody
FFAR2 适用: 人, 小鼠, 大鼠, 猴, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (Extracellular Domain) antibody
FFAR2 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (C-Term) antibody
FFAR2 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 286-315) antibody
FFAR2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 271-320) antibody
FFAR2 适用: 人 ELISA, IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 231-330) antibody
FFAR2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (C-Term) antibody
FFAR2 适用: 人 IHC (p), IF, EIA 宿主: 兔 Polyclonal unconjugated

anti-Free Fatty Acid Receptor 2 (FFAR2) (AA 153-167), (Extracellular) antibody
FFAR2 适用: 人 WB, FACS, LCI 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Kobayashi, Mikami, Kimura, Kamiyama, Morikawa, Yokoi, Kasuno, Takahashi, Taniguchi, Iwano: "Short-chain fatty acids, GPR41 and GPR43 ligands, inhibit TNF-α-induced MCP-1 expression by modulating p38 and JNK signaling pathways in human renal cortical epithelial cells." in: Biochemical and biophysical research communications, Vol. 486, Issue 2, pp. 499-505, (2017) (PubMed).
抗原

FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

别名

GPR43

背景

Synonyms: FFA2R, GPR43, Free fatty acid receptor 2, G-protein coupled receptor 43, FFAR2, FFA2, GPCR43
Background: G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, PubMed:12711604, PubMed:23589301). Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes (PubMed:21037097).

基因ID

2867

UniProt

O15552