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pan Keratin 抗体

This anti-pan Keratin antibody is a 小鼠 单克隆 antibody detecting pan Keratin in IHC (p) 和 IHC (fro). Suitable for 人.
产品编号 ABIN966802
发货至: 中国

Quick Overview for pan Keratin 抗体 (ABIN966802)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This pan Keratin antibody is un-conjugated

应用范围

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

B311-1
  • 特异性

    This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.

    纯化方法

    Concentrated.

    免疫原

    Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

    亚型

    IgG1 kappa
  • 应用备注

    IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
    Western blotting: 1:100-1:400
    Immunoprecipitation.
    Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.

    说明

    Cellular Localization: Cytoplasmic.
    Recommended Positive Control: Skin, Colonic Carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.35 mg/ml.

    缓冲液

    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C
  • 抗原

    pan Keratin (panKRT)

    别名

    Pan Cytokeratin, Pan CK-II

    分子量

    45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
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