pan Keratin 抗体
Quick Overview for pan Keratin 抗体 (ABIN966802)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.
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纯化方法
- Concentrated.
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免疫原
- Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.
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亚型
- IgG1 kappa
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应用备注
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IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections. -
说明
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Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.35 mg/ml.
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缓冲液
- 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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- pan Keratin (panKRT)
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别名
- Pan Cytokeratin, Pan CK-II
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分子量
- 45, 52.5 ,54, 56, 56.5, 58, & 59 kDa
抗原
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