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RFP 抗体 (Biotin)

This anti-RFP antibody is a 小鸡 多克隆 antibody detecting RFP in WB, ELISA, IF 和 FM. Suitable for Discosoma. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN1607901
Supplier Product No.: 600-906-379
发货至: 中国

Quick Overview for RFP 抗体 (Biotin) (ABIN1607901)

抗原

See all RFP 抗体
RFP (Red Fluorescent Protein (RFP))

适用

Discosoma

宿主

  • 20
  • 14
  • 9
  • 7
  • 2
  • 1
小鸡

克隆类型

  • 32
  • 21
多克隆

标记

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  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This RFP antibody is conjugated to Biotin

应用范围

  • 34
  • 23
  • 20
  • 13
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  • 11
  • 11
  • 10
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Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • Supplier Product No.

    600-906-379

    Supplier

    Rockland

    原理

    RFP Antibody Biotin Conjugated

    交叉反应 (详细)

    RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP.

    产品特性

    Synonyms: chicken anti-RFP antibody biotin conjugation, biotin conjugated chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

    纯化方法

    RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above.

    免疫原

    Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

    Immunogen Type: Recombinant Protein

  • 应用备注

    Application Note: Anti-RFP is designed to detect recombinant RFP. This antibody has been tested to detect RFP by immunoblotting and immunofluorescence and is suitable for use in immunohistochemistry. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.

    Western Blot Dilution: 1:1,000 - 1:3,000

    ELISA Dilution: 1:10,000

    IF Microscopy Dilution: 1:100

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度

    1 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." in: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

  • 抗原

    RFP (Red Fluorescent Protein (RFP))

    别名

    DsRed

    背景

    Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including western blotting.

    UniProt

    Q9U6Y8
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