Transferrin 抗体 (Biotin)
Quick Overview for Transferrin 抗体 (Biotin) (ABIN1607870)
抗原
See all Transferrin (TF) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-4634-0100
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Supplier
- Rockland
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交叉反应 (详细)
- Cross reactivities against Transferrin from other sources may occur but have not been specifically determined.
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纯度
- Anti-TRANSFERRIN Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum].
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内毒素水平
- Low Endotoxin : No
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免疫原
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Transferrin [Human Serum]
Immunogen Type: Native Protein -
亚型
- IgG
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应用备注
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Anti-TRANSFERRIN Antibody, Biotin Conjugated has been tested for use in Immunoblotting, ELISA, immunohistochemistry, immunofluorescence microscopy as well as other antibody based assays. Optimal concentrations should be determined by the researcher.
ELISA Dilution: 1:20.000 - 1:150.000
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free -
注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
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有效期
- Expiration date is one (1) year from date of opening.
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- Transferrin (TF)
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别名
- Transferrin
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背景
- Anti-Transferrin Antibody, Biotin Conjugated is specific for the transferrin protein. Transferrins are iron-binding blood plasma glycoproteins which regulate the level of free iron in biological fluids. When free of bound iron, transferrin is referred to as apotransferrin. Human transferrin is encoded by the TF gene and is mainly synthesized in the liver, but other sources, such as the brain produce it. The main role of transferrin is delivery of iron from absorption centers in the duodenum and macrophages to all tissues. Membrane receptors on erythroid precursors in the bone marrow avidly bind transferrin. Hereditary hemochromatosis and anemia are well establish disorders involving defects in transferrin transport defects. Anti-Transferrin Antibody, Biotin Conjugated is suitable for use in cardiovascular research as well as other basic research. Synonyms: Apotransferrin antibody, Beta 1 metal binding globulin antibody, DKFZp781D0156 antibody, PRO1400 antibody, PRO1557 antibody, PRO2086 antibody, Serotransferrin precursor antibody, Siderophilin antibody, TF antibody
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基因ID
- 7018
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UniProt
- P02787
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途径
- Transition Metal Ion Homeostasis
抗原
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