Urease 抗体 (Biotin)
Quick Overview for Urease 抗体 (Biotin) (ABIN1607815)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4682-0100
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Supplier
- Rockland
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原理
- Urease Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
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产品特性
- Synonyms: rabbit anti-Urease Antibody biotin Conjugation, biotin Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
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纯化方法
- Anti-Urease antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Urease [Jack Bean]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Urease (URE) antibody (HRP)
URE 适用: Jack Bean WB, IP, ELISA, DB 宿主: 兔 Polyclonal HRP
anti-Urease (URE) antibodyURE 适用: Jack Bean WB, IP, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Urease biotin conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Urease (URE)
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别名
- Urease
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背景
- Background: Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.
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UniProt
- P07374
抗原
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