Urease 抗体 (HRP)
-
- 抗原 See all Urease (URE) products
- Urease (URE)
-
适用
- Jack Bean
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This Urease antibody is conjugated to HRP
-
应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Urease from other tissues and species may occur but have not been specifically determined.
- 纯化方法
- Urease is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
- 免疫原
-
Immunogen: Urease [Jack Bean]
Immunogen Type: Native Protein
- 亚型
- IgG
-
anti-Urease (URE) antibody
URE 适用: Jack Bean WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
Application Note: Anti-Urease has been assayed against 1.0 μg of Urease [Jack Bean] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- Urease (URE)
- 别名
- Urease (URE 产品)
- 别名
- F12B7.10 antibody, F12B7_10 antibody, urease antibody, urease antibody, URE antibody, CNH01900 antibody
- 背景
-
Synonyms: Urea Amidohydrolase antibody
Background: Urease is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
Gene Name: UREA_CANEN
- UniProt
- P07374
-
