GLUD1 抗体 (Biotin)
Quick Overview for GLUD1 抗体 (Biotin) (ABIN1607789)
抗原
See all GLUD1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4658-0100
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Supplier
- Rockland
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原理
- Anti-Glutamate Dehydrogenase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].
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产品特性
- Synonyms: rabbit anti-Glutamate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1
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纯化方法
- Anti-Glutamate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glutamate Dehydrogenase [Bovine Liver]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glutamate Dehydrogenase 1 (GLUD1) antibody
GLUD1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 54-240) antibodyGLUD1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 兔, 绵羊, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 65-273) antibodyGLUD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 犬, 绵羊, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 182-231) antibodyGLUD1 适用: 人, 小鼠, 大鼠, Cow, 马, Pig, 斑马鱼, 兔, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) antibodyGLUD1 适用: 人 WB, ELISA, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (AA 93-107) antibodyGLUD1 适用: 人, 小鼠, 马, Pig, 猴 WB, IHC, ELISA, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Dehydrogenase 1 (GLUD1) (N-Term) antibodyGLUD1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Glutamate Dehydrogenase Biotin Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))
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别名
- GLUD1
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背景
- Background: Anti-glutamate dehydrogenase antibody recognizes the glutamate dehydrogenase protein. Glutamate dehydrogenase is an enzyme that is present in the mitochondria of eukaryotes. Glutamate dehydrogenase plays a role in urea synthesis by converting glutamate to α-ketoglutarate, and vice versa. Typically, the α-ketoglutarate to glutamate reaction does not occur in mammals as glutamate dehydrogenase equilibrium favors the production of ammonia and α-ketoglutarate. Glutamate dehydrogenase also has a very high affinity for ammonia. Therefore toxic levels of ammonia would have to be present in the body for the reverse reaction to proceed. In bacteria, the ammonia is assimilated to amino acids via glutamate and aminotransferases. In plants, the enzyme can work in either direction depending on environment and stress. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. Anti-glutamate dehydrogenase antibody is suitable for researchers in Cell Biology and Immunology.
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基因ID
- 281785, 32880221
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UniProt
- P00366
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途径
- Positive Regulation of Peptide Hormone Secretion, Warburg Effect
抗原
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