Intestinal Alkaline Phosphatase 抗体 (Biotin)
Our Local Distributor
北京 101111
Quick Overview for Intestinal Alkaline Phosphatase 抗体 (Biotin) (ABIN1607725)
抗原
See all Intestinal Alkaline Phosphatase (ALPI) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-406-142-0100
-
Supplier
- Rockland
-
原理
- ALKALINE PHOSPHATASE Antibody Biotin Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine].
-
产品特性
- Synonyms: Rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
-
纯化方法
- Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
免疫原
-
Immunogen: Alkaline Phosphatase [Human Intestine]
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 397-458) antibody
ALPI 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3B10E7 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 397-458) antibodyALPI 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3B10E7 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 74-162) antibodyALPI 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3A8 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) antibodyALPI 适用: 人 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 20-333) antibodyALPI 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 20-240) antibodyALPI 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 26-500) antibodyALPI 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 397-458) antibodyALPI 适用: 人 ELISA, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3B10E7 unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) (AA 53-102), (pSer87) antibodyALPI 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intestinal Alkaline Phosphatase (ALPI) antibodyALPI 适用: Cow WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Anti-Alkaline Phosphatase Biotin Antibody has been tested by ELISA and dot blot. This product is assayed against 1.0 μg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: User Optimized
ELISA Dilution: User Optimized
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 10 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- Intestinal Alkaline Phosphatase (ALPI)
-
背景
- Background: At a high pH , Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research.
-
基因ID
- 248
-
NCBI登录号
- NP_001622
-
UniProt
- P09923
抗原
-
