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- 抗原 See all ADA 抗体
- ADA (Adenosine Deaminase (ADA))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ADA antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- 交叉反应 （详细）
- Cross reactivity against Adenosine Deaminase from other sources may occur but has not been specifically determined.
- 纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].
- 内毒素水平
- Low Endotoxin : No
- 免疫原
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Adenosine Deaminase [Calf Spleen]
Immunogen Type: Native Protein - 亚型
- IgG
- Top Product
- Discover our top product ADA Primary Antibody
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anti-Adenosine Deaminase (ADA) (AA 1-363) antibody
ADA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-238) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 11-280) antibodyADA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 157-343) antibodyADA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G4-1C6 unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 287-314), (C-Term) antibodyADA 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31396 unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-238) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-241) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This product has been assayed against 1.0 µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15.000 to 1:64.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10 mg/mL
- 缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - 注意事项
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- 有效期
- Expiration date is one (1) year from date of opening.
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- 抗原
- ADA (Adenosine Deaminase (ADA))
- 别名
- Adenosine Deaminase (ADA 产品)
- 别名
- ADA-like antibody, xada antibody, ADA antibody, CG11994 antibody, Dmel\\CG11994 antibody, DrosADA antibody, dADA antibody, zgc:92028 antibody, adenosine deaminase antibody, adenosine deaminase S homeolog antibody, Adenosine deaminase antibody, ADA antibody, Ada antibody, ada.S antibody, ada antibody
- 背景
- Adenosine Deaminase catalyzes the deamination of adenosine to inosine, thus destabilizing double-stranded RNA. Anti-Adenosine Deaminase is a member of the adenosine and AMP deaminases family. This antibody is a factor in purine metabolism and adenosine homeostasis, as well as positively regulating T-cell coactivation through the binding of DPP4. The antibody interacts with DPP4 in the extracellular domain, thus regulating lymphocyte-epithelial cell adhesion. It moderates extracellular adenosine signaling. Enzyme deficiency in Adenosine Deaminase has been associated with severe combined immunodeficiency disease, whereas an overabundance may result in congenital hemolytic anemia. Anti-Adenosine Deaminase is ideal for investigators involved in Enzyme, DNA and RNA research. Synonyms: Adenosine deaminase EC=3.5.4.4 Adenosine aminohydrolase
- 基因ID
- 280712
- UniProt
- P56658
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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