ADA 抗体 (Biotin)
Quick Overview for ADA 抗体 (Biotin) (ABIN1607721)
抗原
See all ADA 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-406-140-0100
-
Supplier
- Rockland
-
原理
- Adenosine Deaminase Antibody Biotin Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].
-
产品特性
- Synonyms: rabbit anti-Adenosine Deaminase Antibody biotin Conjugation, biotin conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase
-
纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
免疫原
-
Immunogen: Adenosine Deaminase [Calf Spleen]
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-Adenosine Deaminase (ADA) (AA 1-363) antibody
ADA 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-238) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 11-280) antibodyADA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 157-343) antibodyADA 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G4-1C6 unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 287-314), (C-Term) antibodyADA 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31396 unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-238) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 1-363) antibodyADA 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Adenosine Deaminase (ADA) (AA 2-241) antibodyADA 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Anti-Adenosine Deaminase Biotin Antibody has been tested by western blot. This product is suitable to be assayed against 1.0 μg of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:64,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 10 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- ADA (Adenosine Deaminase (ADA))
-
别名
- ADA
-
背景
- Background: Adenosine Deaminase catalyzes the deamination of adenosine to inosine, thus destabilizing double-stranded RNA. Anti-Adenosine Deaminase is a member of the adenosine and AMP deaminases family. This antibody is a factor in purine metabolism and adenosine homeostasis, as well as positively regulating T-cell coactivation through the binding of DPP4. The antibody interacts with DPP4 in the extracellular domain, thus regulating lymphocyte-epithelial cell adhesion. It moderates extracellular adenosine signaling. Enzyme deficiency in Adenosine Deaminase has been associated with severe combined immunodeficiency disease, whereas an overabundance may result in congenital hemolytic anemia. Anti-Adenosine Deaminase is ideal for investigators involved in Enzyme, DNA and RNA research.
-
基因ID
- 280712
-
NCBI登录号
- NP_776312
-
UniProt
- P56658
-
途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
抗原
-
