FTH1 抗体 (Biotin)
Quick Overview for FTH1 抗体 (Biotin) (ABIN1607711)
抗原
See all FTH1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
 - 200-406-090-0100
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Supplier
 - Rockland
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原理
 - Ferritin Antibody Biotin Conjugated
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交叉反应 (详细)
 - Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].
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产品特性
 - Synonyms: rabbit anti-Ferritin Antibody Biotin Conjugation, biotin conjugated rabbit anti-Ferritin Antibody, FTH, FTHL6
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纯化方法
 - Anti-Ferritin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Ferritin [Human Spleen]
Immunogen Type: Native Protein
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亚型
 - IgG
 
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Ferritin Biotin antibody has been tested by western blot and is suitable for ELISA and Immunohistochemistry. Expect band at 21.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
 - 10 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 
                                            
储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
 - 4 °C,-20 °C
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储存方法
 - Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
 - 12 months
 
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- FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
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别名
 - FTH1
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背景
 - Background: Ferritin Antibody detects human ferritin. Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The amount of ferritin stored reflects the amount of iron stored. The protein is produced by almost all living organisms, including algae, bacteria, higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is a globular protein complex consisting of 24 protein subunits and is the primary intracellular iron-storage protein in both prokaryotes and eukaryotes, keeping iron in a soluble and non-toxic form. Ferritin that is not combined with iron is called apoferritin. Anti-Ferritin antibody is ideal for investigators involved in enzyme and ubiquitin and UBL research.
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基因ID
 - 2495
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UniProt
 - P02794
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途径
 - Transition Metal Ion Homeostasis
 
抗原
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