IdU 抗体
(1 reference)-
- 抗原 See all IdU products
- IdU (Iododeoxyuridine (IdU))
-
适用
- 化学剂
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This IdU antibody is un-conjugated
- 应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Flow Cytometry (FACS)
- 交叉反应 （详细）
- Cross reactivity is not observed with BrdU, CldU or FdU.
- 纯度
- Anti-IdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody is specific for IdU.
- 内毒素水平
- Low Endotoxin : No
- 免疫原
-
Anti-IdU mononclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Immunogen Type: Other - 克隆位点
- 32D8-D9
-
anti-Iododeoxyuridine (IdU) antibody
IdU 适用: 化学剂 IHC, IF 宿主: 小鼠 Monoclonal OTI2B10 unconjugated
anti-Iododeoxyuridine (IdU) antibodyIdU 适用: 化学剂 IHC, IF 宿主: 小鼠 Monoclonal OTI7C9 unconjugated
anti-Iododeoxyuridine (IdU) antibodyIdU 适用: 化学剂 IHC, IF 宿主: 小鼠 Monoclonal OTI3F3 unconjugated
-
- 应用备注
-
Anti-IdU Antibody has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated IdU thymidine analog from replicated cells.
ELISA Dilution: 1:1000-1:10000
Western Blot Dilution: 1:2000 - 1:5000 - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store IdU Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- Expiration date is one (1) year from date of opening.
-
-
: "Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice." in: International journal of molecular sciences, Vol. 20, Issue 14, (2019) (PubMed).
-
: "Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice." in: International journal of molecular sciences, Vol. 20, Issue 14, (2019) (PubMed).
-
- 抗原
- IdU (Iododeoxyuridine (IdU))
- Abstract
- IdU 产品
- 物质类
- Chemical
- 背景
- Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. Synonyms: Iododeoxyuridine, 5-iodo-2'-deoxyuridine, IdU
-
