Papain 抗体 (Biotin)
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- 抗原
- Papain
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适用
- Papaya
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Supplier Product No.
- 200-1674-0100
- Supplier
- Rockland
- 原理
- Papain Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya].
- 产品特性
- Synonyms: goat anti-Papain Antibody biotin Conjugation, biotin Conjugated goat anti-Papain Antibody, Allergen Car p 1 antibody, Papaya proteinase I antibody, PPI antibody
- 纯化方法
- Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Papain [Carica papaya]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-Papain (AA 134-345) antibody
适用: Papaya ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Papain antibody (HRP)适用: Papaya ELISA, WB, IHC, DB 宿主: 山羊 Polyclonal HRP
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Papain Biotin antibody has been tested by ELISA and western blot. Expect band at 38.9 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:1,000 - 1:10,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Papain
- 背景
- Background: Anti-Papain antibody recognizes the cysteine protease papain commonly found in papaya (Carica papaya). Papain hydrolyzes a broad range of peptides with preference for bulky hydrophobic amino acids at the P2 position. Papain is a single-chained polypeptide stabilized by three disulfide bridges. Its active site contains a catalytic dyad composed of cysteine-25 and histidine-159. Anti-Papain antibody is ideal for researchers interested in Cell Biology and Proteolysis.
- UniProt
- P00784
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