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ATF6 抗体 (AA 437-449)

The 山羊 多克隆 anti-ATF6 antibody (ABIN1590087) specifically detects ATF6 in WB, ELISA, IF 和 FACS. The antibody is reactive with 人 samples.
产品编号 ABIN1590087
发货至: 中国
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Quick Overview for ATF6 抗体 (AA 437-449) (ABIN1590087)

抗原

See all ATF6 抗体
ATF6 (Activating Transcription Factor 6 (ATF6))

适用

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宿主

  • 97
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山羊

克隆类型

  • 86
  • 22
多克隆

标记

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This ATF6 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

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    AA 437-449

    原理

    ATF6 (aa437-449)

    序列

    NSYRYDHSVS NDK

    交叉反应

    Cow, 犬, 人, Pig, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 75-80 kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6 kDa according to NP_031374.2). Recommended concentration: 0.3-2 μg/mL. Primary incubation 1 hour at room

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    ATF6 (Activating Transcription Factor 6 (ATF6))

    别名

    ATF6

    背景

    ATF6, activating transcription factor 6, ATF6A, cAMP-dependent transcription factor ATF-6 alpha, cyclic AMP-dependent transcription factor ATF-6 alpha

    基因ID

    22926, 304962

    NCBI登录号

    NP_031374

    途径

    ER-Nucleus Signaling, Unfolded Protein Response
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