MAPKAP Kinase 2 抗体 (AA 44-55)
Quick Overview for MAPKAP Kinase 2 抗体 (AA 44-55) (ABIN1590004)
抗原
See all MAPKAP Kinase 2 (MAPKAPK2) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 44-55
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原理
- MAPKAPK2 (aa44-55)
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序列
- PQFHVKSGLQ IK
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特异性
- This antibody is expected to recognize both reported isoforms (NP_004750.1, NP_116584.2).
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Western Blot: In transfected HEK293 transiently expressing either Mouse MK2 fused to GFP or the similar Mouse MK5 fused to GFP, only the expected band was observed in the MK2 lane after EB11929 and EB11930 labeling. Data obtained from Dr. M. B. Menon, I
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunoprecipitation: MK2/MK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))
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别名
- MAPKAPK2
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背景
- MAPKAPK2, mitogen-activated protein kinase-activated protein kinase 2, MAPKAP-K2, MK-2, MK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2
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基因ID
- 9261, 17164, 289014
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NCBI登录号
- NP_004750, NP_116584
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途径
- MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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